affigene® Aspergillus tracer

affigene® aspergillus tracer is a CE-labelled in vitro diagnostic assay that utilizes real-time Polymerase Chain Reaction (PCR) amplification for the qualitative determination of Aspergillus spp DNA in human clinical samples. It is intended for the detection of Aspergillus spp DNA in human whole blood and plasma samples.

The kit includes all required reagent for the complete analysis of Aspergillus, from amplification to analysis of results. The reagents are supplied in ready-to-use, single-use format.

The real-time PCR detection procedure is fully licensed and the affigene® aspergillus tracer test profile harmonises with other affigene® assays to enable multi-target runs.

The sensitivity is high in both plasma and whole blood (<0.5 genome equivalents/ul). The kit is highly specific to aspergillus spp DNA and does not cross-react with e.g. Candida Albicans, Exophaila Dermatitis, Malassezia Furfur, and Saccaromyces Cervisiae or any human, viral or bacterial DNA.

False negative results are prevented through a built-in internal control and the risk of getting false negative results with the assay has been determined to <1%. False positive results are prevented through selective UDG amplification, closed-tube reactions and single-use reagents.