Can I re-use the Eluent from the affigene® DNA extraction kit once it has been warmed to 70 degrees?

Can I use the results of the controls (non-template control and positive control) from a previous affigene® tracer run?

Do I need to run the controls (non-template control and positive control) each time, when running an affigene® tracer assay?

Can I use sealing film instead of the clear strip caps to my 96-well plate in the MX3000P?

During extraction I added wash buffer 2 before wash buffer 1, what will happen?

I started a run with the default thermal profile for 7 cycles before I realized my mistake. Is it possible to start all over again with the correct thermal profile and the same PCR reactions?

Do I need to run all three controls (non-template control, calibrator Low and calibrator High) each time, when running an affigene® trender assay?

Can I use the results of the controls (non-template control, calibrator Low and calibrator High) from a previous affigene® trender run?

How do I know that the PCR reaction has been successful?

How many times can the Master Mix be frozen and thawn?

Do I need to work on ice with the Master Mix?

There is a white precipitate in the working lysis buffer, what should I do?

For how long is the working lysis buffer containing internal control (IC) stable?

How can I avoid rough baselines that sometimes occur in the beginning of a run?

How long does it take to perform an assay?

How can I avoid cross-contamination between runs?