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FAQ - Mx3000P/ affigene® software

Q:

I am not able to import the text file from my run. The software reports “A calculation-error occurred: Object reference not set to an instance of an object. No report is available” why?

Q:

Is it possible to “download” data from the Mx3000P instrument after shutting down the instrument and/or the computer?

Q:

The test is not approved in the affigene® analysis software because of failed NTC (non-template control). Can I get a print-out of the results for the negative samples, provided the IC is valid for those samples?

Q:

Why do not all amplification-curves reach the same end fluorescence (dR Last) in the same run?

Q:

I got an error message saying "Test NOT approved" and "Result is not reported as test is not approved". What is wrong?

Q:

I got a message saying "Re-run sample and check manual for more info!"
What does that mean?

Q:

How can I check the life-time of the lamp in the Mx3000P instrument?

Q:

I get an error message saying "A calculation-error occurred: No NTC-well found No report is available". What is wrong?

Q:

I got a bad run with almost no amplification at all and looking at the PCR tubes afterwards most of the liquid is stack up in the lids, what went wrong?

Q:

The software reports a titer and a message ***.
What does that mean?

Q:

The software reports a titer and a message, either * or **.
What does that mean?

Q:

What is the "baseline"?

Q:

The test passes the criteria and all titers are calculated, but there is an error message ("Not as specified") along with the parameters. What is wrong?

Q:

Why can´t I export the results?

Q:

Why can´t I print the results?

Q:

Occasionally I get a message saying "Several testtypes found in data, please load data from one test at a time". What does that mean?

Q:

I couldn´t set the threshold because there was no IC signal for the NTC.

Q:

Why do you assign a fixed Ct value for calibrator High?

Q:

What is the "threshold"?

Q:

I didn´t get amplification in any well.

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